miR-127-5p Targets JAM3 to Regulate Ferroptosis, Proliferation, and Metastasis in Malignant Meningioma Cells.

Objective: Meningiomas are one of the most common primary tumors of the central nervous system. Most of them are benign and can be cured by surgery, while a few meningiomas are malignant. Ferroptosis gene characteristics might be associated with drug therapy and survival in patients with clinically aggressive, unresectable meningiomas. This study explored the mechanism of differentially expressed miRNAs and ferroptosis in meningioma to provide a new reference to treat meningioma. Methods: Bioinformatics analysis of differential miRNA profiles and functions in patients with meningioma was performed. The contents of lactate dehydrogenase (LDH), malondialdehyde (MDA), and Fe2+ were determined. Reactive oxygen species (ROS) values, as well as cell cycle changes, were analyzed by flow cytometry. The targets of miR-127-5p and JAM3 were detected by dual luciferase assays. Cell counting kit-8 (CCK8) and Transwell assays were used to analyze cell activity. Ki67 expression was analyzed by immunohistochemistry. Expression levels of miR-127-5p and JAM3 were analyzed by RT-qPCR. GPX4 expression was quantified by western blotting. Results: miR-127-5p was expressed at low levels in IOMM-Lee cells, while JAM3 was highly expressed in IOMM-Lee cells. A dual luciferase assay demonstrated that miR-127-5p could target JAM3. Upregulation of miR-127-5p in IOMM-Lee cells resulted in cell cycle arrest and inhibition of cell activity. Upregulation of miR-127-5p increased LDH, MDA, and ROS levels and Fe2+ content and inhibited the expression of GPX4 protein. Upregulation of JAM3 reversed the results of miR-127-5p upregulation. Conclusion: miR-127-5p regulated meningioma formation and ferroptosis through JAM3, providing insights for the development of new treatments for meningioma.

[Treatment of multiple or recurrent intracranial tumors with local chemotherapy by hypodermic Ommaya reservoir].

OBJECTIVE: To explore the treatment of multiple or recurrent intracranial tumors by hypodermic placement of Ommaya reservoir and local chemotherapy with methotrexate. METHODS: Retrospective analyses were performed on the clinical data of 45 cases with multiple or recurrent intracranial tumors at Friendship Affiliated Hospital, Capital Medical University from January 2010 to September 2013. All 45 cases had a placement of Ommaya reservoir under skin. Shunt tubes were placed in ventricle (n = 41), cystic tumor cavity (n = 3) and tumor cavity (n = 1). Methotrexate was injected postoperatively into Ommaya reservoir by transcutaneous paracentesis. RESULTS: After local chemotherapy, head magnetic resonance imaging (MRI) re-examinations were performed. Tumors disappeared (n = 32), shrunk significantly (n = 10) and had no significant improvement of symptoms with spinal implantation metastasis (n = 1). During chemotherapy, there were two mortalities due to septic shock and heart failure (n = 1 each). Follow-ups were conducted for 32 cases and 7 of them died. One case had operation-related complications. After an adjustment of shunt tube, symptoms and signs improved. And after local chemotherapy, tumor disappeared on MRI. CONCLUSION: The placement of Ommaya reservoir is mini-invasive and well-tolerated. And local chemotherapy with methotrexate by Ommaya reservoir is convenient and it may reduce systemic side effects, improve the efficacy of chemotherapy drugs, prolong survival time and enhance quality of life.

[Treatment of chronic subdural hematoma by minimally invasive drilling and drainage].

OBJECTIVE: To discuss the treatment of chronic subdural hematoma by minimally invasive drilling and drainage. METHODS: The clinical data were collected and analyzed for 118 cases with chronic subdural hematoma at our hospital from May 2003 to August 2011. They underwent minimally invasive drilling and drainage. RESULTS: A total of 101 cases (85.6%) were cured and 17 cases (14.4%) improved. Hematoma recurred in 6 cases (5.08%). Among them, 5 cases recurred at Months 1-2 and 1 case at Month 77 post-operation. Three cases died. The causes included heart failure (n = 1), cirrhosis and renal failure (n = 1) and sudden cardiac death (n = 1). Six recurrent cases were cured by minimally invasive drilling and drainage. And 85 cases received a follow-up period of 1 - 80 months. No further recurrence was found for 6 recurrent cases. CONCLUSION: The treatment of chronic subdural hematoma by minimally invasive drilling and drainage is safe, simple and efficacious.